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We use two closely related self-renewing epithelial tissues as our model systems, namely skin and mammary gland, to investigate the molecular control of stem cell proliferation, differentiation, and lineage plasticity. We also study how transcription factors expressed in the epidermis regulate epidermal-immune cell signaling to control skin inflammation.
We employ a multidisciplinary approach combining mouse genetics with molecular characterizations. We have shown that chromatin effector protein Pygopus 2 Pygo2 converges with Wnt signaling to regulate multiple developmental processes including that of skin hair follicle and mammary gland.
We have recently identified novel roles of Ovol1 and Ovol2 transcription factors in suppressing epithelial-to-mesenchymal transition in skin and mammary epithelia, laying the ground for us to probe into an uncharted area in skin biology, namely how epithelial plasticity control mechanisms participate in regulating the fates of epithelial stem cells during tissue regeneration.
Haensel D, Dai X. Epithelial-to-mesenchymal transition in cutaneous wound healing: Where we are and where we are heading. MiR promotes mammary stem cell expansion and breast tumorigenesis by suppressing Wnt signaling antagonists.
An Ovol2-Zeb1 mutual inhibitory circuit governs bidirectional and multi-step transition between epithelial and mesenchymal states. Plos Computational Biology 11 Journal of Biological Chemistry Published online on October 18, Developmental Cell, 29 1: Cell Stem Cell, 13 1: Cell Cycle 11 1: Pygo2 expands mammary progenitor cells by regulating histone H3 K4 methylation.
Chosen for news and commentary. Ovol2 suppresses cell cycling and terminal differentiation of keratinocytes by directly repressing c-Myc and Notch1.
Nuclear regulator PYGO2 controls spermiogenesis and chromatin modification. Developmental Biology 2: Analysis of mPygo2 mutant mice suggests a requirement for mesenchymal Wnt signaling in pancreatic growth and differentiation.
Developmental Biology, 2: Ovol1 represses transcription by competing for DNA binding with the c-Myb activator and by recruiting histone deacetylases. Nucleic Acids Research 35, Wnt5a functions in planar cell polarity regulation in mice. Developmental Biology Ovol1 regulates the growth arrest of embryonic epidermal progenitor cells and represses c-Myc transcription.
Cell Biology The mouse Ovol2 gene is required for cranial neural tube development. Ovol1 regulates meiotic pachytene progression during spermatogenesis by repressing Id2 expression.genes and signaling pathways involved in proliferation and differentiation of mammary epithelial cells Dissertation Thesis submitted in fulfillment of the requirements for the degree on my results and helpful criticism of my thesis manuscript.
As proliferation of the ectoderm cells and mesenchymal cells continues, the mammary band develops into mammary streak, mammary hillock, and ultimately into mammary bud.
Mammary band developed at 9 mm CRL in sheep, 5 mm CRL in goat, and 14 mm CRL in cattle [ 17 ]. thesis in mammary epithelial cells is greaterthanthatseenwith either steroid alone (Figure 2B).
The growth elicited in response to both estrogen and progest-erone is characterized by outgrowth from terminal and lateral walls of the ducts and branches, the extent of which.
cytokine CD14 in HME-hTERT cells treated with vitamin D. CD14 is a component of the innate immune system which also functions in apoptotic cell clearance and mammary gland remodeling.
The studies described in the first part of this thesis were designed to confirm and extend this preliminary data. Collagen-binding proteins were studied in mammary epithelial cells of 7,dimethylbenz(a)anthracene-induced rat mammary tumors.
Using affinity chromatography on type I collagen-Sepharose and polyacrylamide slab gel electrophoresis, three major proteins of 34,, 36,, and 38, Da .
i Determinants of cell cycle progression in human mammary epithelial MCF12 cells Alexandra Ouertani Thesis submitted for the degree of Doctor of Philosophy.